An Uber spokeswoman said that today's filing in the Westminster Magistrates' Court is more a formal notification that the appeal is underway and that the company may never appear in court if a settlement is reached.
A hearing is likely to take place on December 11, a spokesman at Britain's Judicial Office told Reuters.
Last month, Transport for London (TfL) refused Uber a new private hire licence, saying the ride-hailing firm was not fit and proper.
Uber on Friday filed its appeal against a decision by London authorities not to renew its licence, the U.S. ride-hailing company said.
It said in a statement it was hoping to "continue having constructive discussions with Transport for London".
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Uber has been in London since 2012.
"As our new CEO has said, we are determined to make things right". The pair are said to have met on 3 October, and both sides have since described the talks as "constructive".
His predecessor, Travis Kalanick, left the company following allegations that managers didn't adequately address reports of sexual harassment.
If Uber loses its appeal, 40,000 jobs will be on the line and although Uber and Londoners believe the company should be allowed to stick around, other members of the UK Government disagree.
More than 850,000 people have signed an online petition launched by Uber, urging TfL to reverse its ruling.
Its spokesman added that details of Uber's arguments against the decision will not be released until nearer the time of the appeal being heard.